reading frame shift

By sequence alignment to Y53 ( 4L), a 13 bp repeat sequence found in exon 3 locus of Y53 ( 14L) caused reading frame shift and the alteration of amino acid sequence.

通过序列比对，结果表明与Y53（4L）相比较，Y53（14L）在外显子3有一段13bp的重复序列，造成ORF的改变，进而引起氨基酸序列改变。

Further DNA sequencing manifested that the insertion element was tsbp sequence with complete reading frame and no frame shift.

进一步DNA测序结果显示插入片段与tsbp编码序列一致，阅读框架完整，并且无移码。

A one base deletion is discovered in purA gene from inosine producing strain and guanosine producing strain, which will cause the open reading frame shift mutation.

分析结果表明两株生产菌的purA基因发生了1个碱基缺失，导致阅读框发生移码突变；

Results Restriction enzyme digestion and DNA sequencing suggested that the recombinant expression plasmid contained correct encoding region of Pokemon, without reading frame shift.

结果限制性核酸酶切及序列分析表明重组表达质粒包含Pokemon基因编码区，阅读框架无移位；